噬菌体Bp7耐受株K12-R生物学特性分析

doi:10.11843/j.issn.0366-6964.2018.02.019

Abstract

Abstract:

E. coli K12 was the host of phage Bp7, and its mutant strain K12-R was resistant to phage Bp7. To understand the resistance reason and the mutation effect on biological properties of K12-R, the genome of K12-R was sequenced and compared with that of E. coli K12 to find the mutation sites, the growth ability and the autoaggregation ability of K12-R were measured by turbidity test and the biofilm formation ability was detected by gram staining method, the biological properties of K12-R were compared with E. coli K12. The results showed that a mutant gene hldE, which was associated with Lipopolysaccharide synthesis, in K12-R caused the resistance to phage Bp7. Compared with E. coli K12, the growth ability of K12-R was reduced, but the cell membrane permeability, the autoaggregation ability and the biofilm formation ability were enhanced. The hldE gene of E. coli K12 was associated with LPS synthesis, the mutation of hldE gene changed the LPS character of K12-R, which caused the resistance to phage Bp7. The results provides the basis to understand the tolerance mechanism of K12-R to phage Bp7.

CLC Number:

S852.6

CHEN Pei-pei, QI Xin, WANG Wei, REN Hui-ying, LIU Wen-hua, ZHANG Can. Biological Analysis of a Phage Bp7 Resistant Strain K12-R[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(2): 396-400.

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Biological Analysis of a Phage Bp7 Resistant Strain K12-R

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